首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   794篇
  免费   83篇
  国内免费   352篇
  2024年   3篇
  2023年   55篇
  2022年   50篇
  2021年   72篇
  2020年   38篇
  2019年   58篇
  2018年   47篇
  2017年   40篇
  2016年   38篇
  2015年   22篇
  2014年   42篇
  2013年   68篇
  2012年   36篇
  2011年   36篇
  2010年   23篇
  2009年   46篇
  2008年   56篇
  2007年   59篇
  2006年   51篇
  2005年   52篇
  2004年   37篇
  2003年   39篇
  2002年   16篇
  2001年   26篇
  2000年   14篇
  1999年   21篇
  1998年   23篇
  1997年   15篇
  1996年   16篇
  1995年   18篇
  1994年   16篇
  1993年   12篇
  1992年   15篇
  1991年   12篇
  1990年   15篇
  1989年   5篇
  1988年   3篇
  1987年   3篇
  1986年   4篇
  1985年   3篇
  1984年   9篇
  1983年   3篇
  1982年   5篇
  1979年   2篇
  1978年   1篇
  1976年   1篇
  1975年   1篇
  1973年   1篇
  1958年   1篇
排序方式: 共有1229条查询结果,搜索用时 93 毫秒
1.
Case histories and proposed mechanisms formicrobiologically influenced corrosion of metals andalloys by metal depositing microorganisms arereviewed. Mechanisms with indirect participation ofthese microorganisms, usually iron- and manganeseoxidizing species, are distinguished from anothermechanism which accounts specifically for theelectrochemical properties of deposits containingoxides and hydroxides of Mn in higher oxidationstates. The possible influence of such deposits whichwere formed microbiologically is evaluated. Theevaluation is based on the principles ofelectrochemical corrosion of metals and on theelectrochemical properties of Mn3+/4+- compounds.After briefly reviewing the microbiologicalMn-oxidation, experimental evidence for the predictedcorrosion by such deposits is provided and a model formicrobiologically influenced corrosion by manganeseoxidizing microorganisms is proposed for stainlesssteel. Possible consequences of the model andpractical aspects of such a corrosion are discussed.  相似文献   
2.
3.
Aim We investigate the long‐standing question of whether the small size of microbes allows most microbial species to colonize all suitable sites around the globe or whether their ranges are limited by opportunities for dispersal. In this study we use a modelling approach to investigate the effect of size on the probability of between‐continent dispersal using virtual microorganisms in a global model of the Earth’s atmosphere. Location Global. Methods We use a computer model of global atmospheric circulation to investigate the effect of microbe size (effective diameters of 9, 20, 40 and 60 μm) on the probability of aerial dispersal. Results We found that for smaller microbes, once airborne, dispersal is remarkably successful over a 1‐year period. The most striking results are the extensive within‐hemisphere distribution of virtual microbes of 9 and 20 μm diameter and the lack of dispersal between the Northern and Southern Hemispheres during the year‐long time‐scale of our simulations. Main conclusions Above a diameter of 20 μm wind dispersal of virtual microbes between continents becomes increasingly unlikely, and it does not occur at all (within our simulated 1‐year period) for those of 60 μm diameter. Within our simulation, the success of small microbes in long‐distance dispersal is due both to their greater abundance and to their longer time in the atmosphere – once airborne – compared with larger microbes.  相似文献   
4.
【目的】从兰州唐古特白刺根际分离得到对植物有潜在促生效果的功能微生物,为研发相关菌种制剂的研究奠定基础。【方法】通过平板划线法从其根际分离纯化出6株细菌,并对菌株进行形态特征观察、革兰氏染色等一系列生理生化试验。用藜麦检测各菌株的促生功能,并对具有优良促生作用的1个菌株16S rRNA基因进行分子鉴定及基因草图绘制。【结果】根据生化鉴定结果,6株细菌分别属于不动杆菌属(Acinetobacter)、土壤杆菌属(Agrobacterium)、类芽孢杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、鞘氨醇单胞菌属(Sphingomonas)和假单胞菌属(Pseudomonas)。其中,16S rRNA基因鉴定BC4属于肠杆菌属(Enterobacter),具有较好的促生效果。【结论】BC4具有较好的促生效果,为兰州唐古特白刺菌种资源的开发和利用提供了一定的理论依据。  相似文献   
5.
Transgenic plants and biogeochemical cycles   总被引:13,自引:0,他引:13  
  相似文献   
6.
Heavy metals are found in their different forms in the environment. The distribution, mobility, and toxicity of metals are strongly related to these different forms. This necessitates the exploration of different methods for the remediation and speciation of heavy metals. Some direct and indirect physico-chemical methods such as filtration, chemical precipitation, ion-exchange, electro deposition, and membrane systems have been used for the last four decades. However, it is only in last few years that reliable biological methods have also been used. The biological methods include the use of microorganisms (fungi, algae, bacteria), plants (live or dead) and biopolymers. The use of these methods for the speciation of heavy metals is reviewed here.  相似文献   
7.
Changes in soil structure and in microbial population were recorded in a long term field experiment over the growing season of maize (June–November). Determinations were made on samples from plots which had received, for two years, the following treatments: mineral fertilizers, farmyard manure and three rates of compost. Seasonal variations were observed for the stability of soil aggregates, total porosity, pore size distribution, mycorrhizal infection and aerobic cellulolytic microorganisms. The stability of the soil aggregates changed in a similar way to that found for both mycorrhizal infection and the number of aerobic cellulolytic microorganisms. Physical characteristics were not affected in any instance by the organic dressings and microbiological populations were generally influenced only by the higher doses of compost.  相似文献   
8.
人工沟渠的生态环境效应研究综述   总被引:10,自引:1,他引:9  
郗敏  吕宪国  刘红玉 《生态学杂志》2005,24(12):1471-1476
人工沟渠主要指以排水和灌溉为目的人工挖掘的水道.人工沟渠作为排水和引水的廊道改善了土壤的结构、促进了作物的生长,从而提高了农作物的产量;同时人工沟渠这种景观的存在对周围的环境也产生了巨大的影响.水文效应是被大家普遍接受的生态环境效应.在整个降雨期间或之后,观察显示出沟渠对出口径流的贡献:一些沟渠收集和向河流输送水;另一些沟渠收集水但是减慢了水流的速度.本文从水文效应、对元素地球化学循环、生物及其生境的影响到对生态环境整体影响方面总结了人工沟渠的生态环境效应,并对其进一步的研究提出一些建议,旨在最大限度发挥人工沟渠的生态环境效应,改善整个生态环境.  相似文献   
9.
Cold-adapted microorganisms are potentially interesting for use in environmental biotechnology applications since a large part of the biosphere has low temperatures during at least parts of the year. Many studies have shown that both oil-contaminated and uncontaminated soils in the Arctic, the Antarctic and the Alps contain microbes that can degrade different hydrocarbons deriving from oils. A few studies have also been conducted on degradation of herbicides in soils at low temperatures. Furthermore, phenols and some polychlorinated biphenyl (PCB) congeners have proved to be degradable at low temperatures, using microorganisms isolated from sediments or soils. Additions of nitrogen and phosphorous to polluted soils have been shown to enhance the degradation of hydrocarbons in many cases. Bioaugmentation with hydrocarbon degrading cold-adapted microorganisms has given varying results. The inoculated microorganisms have probably been out-competed by the indigenous microorganisms in some cases. Different ways to increase the efficiency of microbial degradation of organic pollutants in soil in a cold climate is discussed.  相似文献   
10.
通过特异PCR扩增和16SrDNA序列分析检测动弯杆菌   总被引:5,自引:1,他引:4  
细菌性阴道病(Bacterial Vaginosis,BV)是由于细菌过度生长所致阴道微生态非正常改变,从而导致的一类多微生物病(Polymicrobial Diseases)。动弯杆菌(Mobiluncus sp.)与BV发生有密切关系,但该菌为厌氧菌,营养要求苛刻,很难进行纯培养,国内鲜有研究报道。本文先对BV动物模型恒河猴阴道分泌物进行厌氧菌混合培养,抽提混合物染色体DNA,之后设计了一对动弯杆菌16SrRNA基因的特异性引物,用PCR的方法扩增出了特异片段。通过对扩增产物进行测序分析,确定检测出的为动弯杆菌,并且与羞怯动弯杆菌极为相似。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号